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In the present study, the effect of exogenous tumour necrosis factor alpha (TNF-alpha) on the transition from the germinal vesicle to metaphase II (MII) and the developmental competence of pig embryos was investigated. The resumption of nuclear maturation and MII formation of oocytes from pre-antral follicles was evaluated as the first step and was performed in the presence of 0, 100, 250 or 500 ng/ml TNF-alpha. Then, the treated oocytes were fertilized and cultured to assess the fertilization and early embryo development. The percentages of oocytes that resumed meiosis and formed MII-stage oocytes were significantly lower than in the control. The percentage of oocytes that resumed meiosis was significantly lower with higher TNF-alpha concentrations. A significantly lower blastocyst rate was observed in oocytes cultured from pre-antral follicles after injection of 250 or 500 ng/ml TNF-alpha. Further investigation is required to determine whether TNF-alpha has deleterious effects on the development of pig oocytes to the blastocyst stage.Q:
Converting an old code to MVVM
I would like to do a project in WPF, the old project is using simple code that use DataGrid (with a ObservableCollection). I did some research and I found this article that show MVVM for WPF. I tryed to implement but I’m having some difficulties (coming from other languages, like Java or Objective-C).
Is there a complete tutorial or example of this kind of project (that uses another style than the MVVM style), that could help me develop the whole application on this style?
A:
There are plenty of tutorials that can teach you how to create a simple MVVM project. I don’t know if this is what you mean by „style“, but here’s the MSDN info on how to do MVVM.
Creating a WPF application using the MVVM Model
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