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Schwann cells (SC) are glial cells that regulate the function of axons through a variety of interactions. They provide trophic and cytoprotective support and guide axons in a concentration-dependent manner. There are a number of in vitro assays used to test the toxicological, pharmacological, and other properties of test compounds on SC. Most of these techniques are rather slow and cumbersome, since they are based on the growth and development of SC derived from rodents. A novel cell culture system was developed here that allows more rapid, robust, and functional studies of the responses of these cells to various test compounds. This method is based on the establishment of a short-term culture of primary rat SC in media containing l-ascorbic acid and transforming growth factor beta1 (TGF-beta1). When induced by TGF-beta1, SC cease proliferating and resume a differentiation process that results in the expression of myelin genes. These SC-derived cells resemble SC in vivo by immunocytochemistry as well as their electrophysiological properties. This system permits the rapid and quantitative assessment of SC responses to test compounds. Using this system, the effects of test compounds, including those from natural products, on SC can be studied. The elucidation of these mechanisms may be of value in the development of test compounds, agents used for the treatment of various neuropathologies, and foods of possible neuropharmacological value.Zhang Caiyun

Zhang Caiyun (, born November 1956) is a Chinese human rights activist, human rights activist, and journalist. She is the director of the Beijing-based National Office for the Promotion of Human Rights.

Biography
Zhang was born in November 1956 in Liaoyang, Liaoning, the daughter of a factory worker. She graduated from Beijing Normal University and received her master’s degree from Department of East Asian Languages and Culture in the University of Hawaii in 1980. She returned

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