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channaphilmiAlternative splicing of exon 15 in the chicken fascin gene controls cell migration by switching between two actin-bundling proteins in endothelial cells.
Vascular development depends on the interaction between endothelial cells (EC) and their underlying extracellular matrix (ECM). We have investigated the transcriptional regulation and alternative splicing of the chicken fascin gene, which encodes an actin-bundling protein. Chick fascin mRNA is highly expressed in migrating blood vessels in early embryos. Furthermore, differential splicing of exon 15 occurs between 16 and 30 hr of incubation, and the two splicing variants result in two isoforms of chicken fascin proteins that are distinguished by their N-terminal actin-bundling domains. Transient expression of the two fascin isoforms in cultured cells revealed that the „long“ isoform is selectively involved in the formation of stress fibers and stress fiber-bearing lamellae. However, the „short“ isoform promotes cell migration that depends on lamellipodia formation, which is induced by de novo actin polymerization. The „long“ fascin isoform is functionally switched from the „short“ form by alternative splicing of exon 15. This represents the first identification of molecular mechanisms to regulate alternative splicing of a transcriptional regulator in a specific cell type, and the first functional switch between fascin isoforms, which provides a basis for novel concepts concerning the control of cell migration.Shane Wiebe

Shane Wiebe (born December 31, 1986) is a Canadian professional ice hockey player. He is currently playing with Fischtown Pinguins of the DEL2 in the Deutsche Eishockey Liga (DEL).

Playing career
Wiebe was selected by the Edmonton Oilers in the 7th round (194th overall) of the 2006 NHL Entry Draft after the departure of Steve MacIntyre. He played for the Idaho Steelheads of the ECHL for the 2009–10 season, before he was signed to an AHL contract with the Bakersfield Condors on July 17, 2010.

On July 14, 2011, Wiebe signed a one-year contract with the Alberta Golden Bears of the WHL, and was assigned to the team’s ECHL affiliate, the Louisiana IceGators. In the 2011–12 season, after going pointless for seven games, Wiebe was loaned to the Bakersfield

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Exchange of Ideas and Proceedings of the Second International Symposium on CIFS-related Technology Vigyan Bhavan, New Delhi and National Institute of Science Education and Research, .
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Tricks to not falling asleep in class: 1-breathe through your mouth.
Sleep Pills: How many should I take?
Sleep Clock: How I track my sleep.
The People Like Me: How to get the love of your life.
Did you know that sleeping well is essential to good health? A good night’s rest helps support your body’s immune system, helps you to metabolize, and give you a clear mind to focus on the tasks at hand. If you want to help your body function better, try this movement first: Belly Breathing.
7 Tips to Start Belly Breathing.
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12 Ways to Start Belly Breathing.
Why and when to move.
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